5Z8X
Crystal structure of human LRRTM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.691, 240.959, 259.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.429 - 3.150 |
| R-factor | 0.2042 |
| Rwork | 0.203 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a5c |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 48031 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 96.3 | 88.1 |
| Redundancy | 6.4 | 3.2 |
| CC(1/2) | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 18% PEG 3350, 0.1 M ammonium sulfate, 0.1 M sodium malonate |
