5Z8O
Structural of START superfamily protein MSMEG_0129 from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 62 |
| Unit cell lengths | 109.760, 109.760, 56.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| Rwork | 0.210 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tfz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.785 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Rosetta |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.077 | 0.622 |
| Number of reflections | 55131 | |
| <I/σ(I)> | 19.12 | 3.6 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 7.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 2.0 M sodium formate, 0.1 M MES, pH 6.5 |
