5Z8J

Human mitochondrial ferritin mutant - E27C/E62C/C102G/C130G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-03-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	F 4 3 2
Unit cell lengths	182.654, 182.654, 182.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.904 - 2.300
R-factor	0.181
Rwork	0.180
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r03
RMSD bond length	0.007
RMSD bond angle	0.797
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.904	2.383
High resolution limit [Å]	2.300	2.300
Number of reflections	12124
<I/σ(I)>	7.1
Completeness [%]	100.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	297	3.4M 1,6-Hexanediol, 0.1M Tris-HCl