5Z82
Structural basis for specific inhibition of highly sensitive ShHTL7 receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 73.980, 84.040, 90.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.090 - 1.694 |
R-factor | 0.1955 |
Rwork | 0.194 |
R-free | 0.22050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cbk |
RMSD bond length | 0.007 |
RMSD bond angle | 0.791 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.280 | 1.760 |
High resolution limit [Å] | 1.690 | 1.700 |
Number of reflections | 60066 | |
<I/σ(I)> | 12.05 | |
Completeness [%] | 99.8 | |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, Magnesium Chloride, Tris-HCl pH 8.5 |