5Z7N
SmChiA sliding-intermediate with chitopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 131.110, 200.360, 59.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.700 |
| Rwork | 0.173 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eib |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.098 | 0.731 |
| Number of reflections | 164036 | 26031 |
| <I/σ(I)> | 12.7 | 2.52 |
| Completeness [%] | 98.8 | 96.8 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 294 | 700 mM Sodium citrate, 10% methanol, 5% glycerol |
