5Z7L

Crystal structure of NDP52 SKICH region in complex with NAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-06
Detector	AGILENT EOS CCD
Wavelength(s)	0.97914
Spacegroup name	C 1 2 1
Unit cell lengths	113.639, 45.026, 86.679
Unit cell angles	90.00, 123.77, 90.00

Refinement procedure

Resolution	40.645 - 2.020
R-factor	0.1727
Rwork	0.170
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.852
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.050	2.070
High resolution limit [Å]	2.020	2.020
Number of reflections	24065
<I/σ(I)>	10.6
Completeness [%]	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG3350,sodium malonate