5Z7D

p204HINab-dsDNA complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	101.390, 101.390, 783.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.260 - 4.500
R-factor	0.2617
Rwork	0.260
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yzw 5yzp
RMSD bond length	0.004
RMSD bond angle	0.708
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.660
High resolution limit [Å]	4.500	4.500
Rmeas	0.099
Number of reflections	14163	15350
<I/σ(I)>	11.8	0.85
Completeness [%]	99.5	99.3
Redundancy	12.8	13.4
CC(1/2)	0.999	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	4% PEG8000, 100mM KCl, 100mM MES pH 6.5