5Z76
Artificial L-threonine 3-dehydrogenase designed by full consensus design
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.460, 93.540, 176.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.884 - 2.800 |
| R-factor | 0.2156 |
| Rwork | 0.215 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.900 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.107 | 0.417 |
| Number of reflections | 38858 | |
| <I/σ(I)> | 15.6 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 3350, 0.2M ammonium citrate dibasic |
