5Z74
Crystal structure of alkaline/neutral invertase InvB from Anabaena sp. PCC 7120 complexed with sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.946, 82.171, 97.446 |
| Unit cell angles | 90.00, 119.13, 90.00 |
Refinement procedure
| Resolution | 31.350 - 1.950 |
| R-factor | 0.20978 |
| Rwork | 0.208 |
| R-free | 0.23854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z73 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.114 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.096 | 0.537 |
| Number of reflections | 80206 | 8173 |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 95.7 | 98.1 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.7M lithium chloride, 6% polyethylene glycol 6000, 0.1M MES, pH 6.0 |
