5Z73
Crystal structure of alkaline/neutral invertase InvB from Anabaena sp. PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.637, 82.384, 94.829 |
| Unit cell angles | 90.00, 118.86, 90.00 |
Refinement procedure
| Resolution | 31.410 - 1.930 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.22767 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.914 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.078 | 0.505 |
| Number of reflections | 82269 | 8274 |
| <I/σ(I)> | 15.1 | 2.3 |
| Completeness [%] | 97.6 | 98.9 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.7M lithium chloride, 6% polyethylene glycol 6000, 0.1M MES, pH 6.0 |
