5Z6N
Crystal structure of Escherichia coli ElaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.149, 40.905, 84.648 |
| Unit cell angles | 90.00, 108.30, 90.00 |
Refinement procedure
| Resolution | 30.046 - 2.600 |
| R-factor | 0.2791 |
| Rwork | 0.278 |
| R-free | 0.30360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jph |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 | 0.336 |
| Rmeas | 0.084 | 0.370 |
| Rpim | 0.035 | 0.150 |
| Number of reflections | 14550 | 691 |
| <I/σ(I)> | 25.1 | 4.3 |
| Completeness [%] | 94.6 | 97.6 |
| Redundancy | 5.8 | 5.7 |
| CC(1/2) | 0.983 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 0.2M Potassium fluoride, 24%(w/v) PEG3350 |
