5Z6C
Crystal structure of Bacillus subtilis sugar-binding protein YesO involved in import of rhamnogalacturonan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.184, 108.578, 40.494 |
| Unit cell angles | 90.00, 101.58, 90.00 |
Refinement procedure
| Resolution | 37.261 - 1.970 |
| R-factor | 0.2025 |
| Rwork | 0.199 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r6k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.120 | 0.278 |
| Number of reflections | 42265 | |
| <I/σ(I)> | 12.1 | 3.06 |
| Completeness [%] | 98.9 | 98.3 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MIB (sodium malonate, imidazole, boric acid) buffer (pH 6.0), 25% (w/v) PEG-1500 |
