5Z5Y
Crystal structure of ConA-R4M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9716 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.600, 116.110, 58.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.000 - 1.890 |
R-factor | 0.19077 |
Rwork | 0.189 |
R-free | 0.24387 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.926 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.900 | 1.940 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 34408 | |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.7 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 300 | 20% polyethylene glycol 6000, 20 mM HEPES, 100 mM NaCl, 5 mM CaCl2, 5 mM MnCl2 pH 7.2 |