5Z59

Crystal structure of Tk-PTP in the inactive form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2017-03-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	29.473, 50.638, 78.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.032 - 1.703
R-factor	0.1899
Rwork	0.186
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i6i
RMSD bond length	0.007
RMSD bond angle	0.905
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	13111
<I/σ(I)>	46
Completeness [%]	97.2
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium citrate (pH 5.4), 8%(w/v) polyethylene glycol 10000, 14%(v/v) dioxane