5Z4M

Structure of TailorD343A with bound UTP and Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	61.962, 84.556, 149.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.981 - 1.740
R-factor	0.1692
Rwork	0.167
R-free	0.20610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nkt
RMSD bond length	0.010
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.770
High resolution limit [Å]	1.740	4.720	1.740
Rmerge	0.071	0.046	0.590
Rmeas	0.074	0.048	0.617
Rpim	0.020	0.014	0.175
Number of reflections	40441	2164	1964
<I/σ(I)>	6.3
Completeness [%]	99.7	98.9	98.9
Redundancy	12.9	12.3	11.5
CC(1/2)		0.998	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Sodium chloride, 0.1M Tris pH 8.4, 15% w/v PEG 3350