5Z4J

Structure of Tailor in complex with U4 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	61.633, 84.840, 148.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.077 - 1.820
R-factor	0.1571
Rwork	0.155
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nkt
RMSD bond length	0.010
RMSD bond angle	1.029
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.850
High resolution limit [Å]	1.820	4.940	1.820
Rmerge	0.083	0.050	0.478
Rmeas	0.087	0.053	0.497
Rpim	0.024	0.015	0.134
Number of reflections	35229	1902	1717
<I/σ(I)>	7.2
Completeness [%]	99.6	98.9	99.7
Redundancy	13.1	12.1	13.4
CC(1/2)		0.998	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Lithium chloride, 0.1M Tris pH 8.4, 15% w/v PEG 3350