5Z4G

Crystal structure of secretory abundant heat soluble protein 4 from Ramazzottius varieornatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-08
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 1
Unit cell lengths	32.802, 41.926, 53.483
Unit cell angles	89.97, 80.75, 78.76

Refinement procedure

Resolution	50.010 - 1.500
R-factor	0.16161
Rwork	0.159
R-free	0.20308
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xna
RMSD bond length	0.007
RMSD bond angle	1.224
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.036	0.260
Rmeas	0.051	0.368
Rpim	0.036	0.260
Number of reflections	42396	2099
<I/σ(I)>	23.1	2.9
Completeness [%]	95.9	95.5
Redundancy	2	2
CC(1/2)		0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	50mM HEPES pH 7.8, 1mM ZnSO4, 24%(v/v) PEG600