5Z49

Crystal structure of the effector-binding domain of Synechococcus elongatus CmpR in complex with ribulose-1,5-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97914
Spacegroup name	P 21 21 21
Unit cell lengths	62.570, 86.700, 87.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.350 - 2.148
R-factor	0.1908
Rwork	0.188
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y2v
RMSD bond length	0.008
RMSD bond angle	1.148
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.890	1.890
Number of reflections	26386
<I/σ(I)>	14.68
Completeness [%]	99.3
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1 M Tris pH 8.5, 25% tert-butanol