5Z42
Aquifex aeolicus MutL endonuclease domain with three zinc ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 35.623, 35.623, 167.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.793 - 1.300 |
| R-factor | 0.1905 |
| Rwork | 0.189 |
| R-free | 0.20410 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.074 | 0.828 |
| Rmeas | 0.077 | 0.859 |
| Rpim | 0.021 | 0.226 |
| Number of reflections | 27699 | 1360 |
| <I/σ(I)> | 50.7 | 3.333 |
| Completeness [%] | 98.9 | 97.7 |
| Redundancy | 13.6 | 14 |
| CC(1/2) | 0.978 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100mM sodium acetate, 5mM zinc acetate, 15%(v/v) polyethylene glycol 400, pH 4.6 |
