5Z3S
Crystal structure of butanol modified signaling protein from buffalo (SPB-40) at 1.65 A resolution
Replaces: 5YARReplaces: 4S19Replaces: 5WSJExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.142, 66.553, 106.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.370 - 1.650 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.20582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5YAR |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.370 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.309 | |
| Number of reflections | 52856 | |
| <I/σ(I)> | 5.8 | 5.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 35mM Tris-HCl, 25mM NaCl, 20% ethanol, pH 8.0 |
