5Z3K
Crystal structure of glucosidase from Croceicoccus marinus at 1.8 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.911, 95.911, 100.908 |
| Unit cell angles | 90.00, 98.71, 90.00 |
Refinement procedure
| Resolution | 48.347 - 1.802 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.19370 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.828 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 82629 | |
| <I/σ(I)> | 15.03 | |
| Completeness [%] | 97.3 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2M Ammonium acetate, 0.1M Bis-Tris pH 5.5, 25% PEG 3350 |
