5Z3A
Glycosidase Wild Type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 104.743, 104.743, 89.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.842 - 1.401 |
| R-factor | 0.1171 |
| Rwork | 0.116 |
| R-free | 0.13970 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.490 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 97738 | |
| <I/σ(I)> | 26.69 | |
| Completeness [%] | 99.9 | |
| Redundancy | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Ammonium citrate tribasic (pH 6.8), 0.1M Sodium citrate (pH 5.5), 12% PEG monomethyl ether 2000, 2mM glucose |
