5Z33

Crystal structure of Mitogen-activated Protein Kinase Mps1 in Magnaporthe oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	74.549, 74.549, 158.561
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.795 - 1.990
R-factor	0.1886
Rwork	0.187
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.280	2.100
High resolution limit [Å]	1.990	1.990
Number of reflections	379824
<I/σ(I)>	12.8
Completeness [%]	99.9
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Sodium cacodylate trihydrate, tacsimate, spermine