5Z2W
Crystal structure of the bacterial cell division protein FtsQ and FtsB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 63.557, 39.306, 73.191 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 36.469 - 3.000 |
| R-factor | 0.2141 |
| Rwork | 0.207 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.970 |
| High resolution limit [Å] | 2.920 | 7.920 | 2.920 |
| Rmerge | 0.084 | 0.030 | 0.980 |
| Rmeas | 0.088 | 0.035 | |
| Rpim | 0.047 | 0.018 | 0.553 |
| Number of reflections | 8124 | 439 | 409 |
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 4.1 | 3.7 | 4 |
| CC(1/2) | 0.998 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 296 | 15% PEG6000, 0.2M magnesium chloride, 0.1M Tris-HCl pH 8.0 |
