5Z2J
structure of S38A mutant metal-free periplasmic metal binding protein from candidatus liberibacter asiaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.070, 94.070, 94.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.470 - 1.870 |
| R-factor | 0.15953 |
| Rwork | 0.158 |
| R-free | 0.19151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4udn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.904 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.480 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 40167 | |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 97.9 | 85.6 |
| Redundancy | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277.15 | 0.1M SODIUM ACETATE TRIHYDRATE PH 4.6, 2M AMMONIUM SULPHATE |
