5Z2H

Structure of Dictyostelium discoideum mitochondrial calcium uniporter N-terminal domain(DdMCU-NTD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	42.159, 59.363, 70.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.504 - 1.674
R-factor	0.1918
Rwork	0.190
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.480	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.050	1.264
Rmeas	0.053	1.320
Rpim	0.016	0.374
Number of reflections	39046	1029
<I/σ(I)>	25.5	2.2
Completeness [%]	99.7	99.7
Redundancy	11.8	12.3
CC(1/2)	0.999	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20%(v/v) PEG 4000, 10%(v/v) 2-propanol, 0.01M HEPES, pH 7.5