5Z2H
Structure of Dictyostelium discoideum mitochondrial calcium uniporter N-terminal domain(DdMCU-NTD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.159, 59.363, 70.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.504 - 1.674 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.497 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.480 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.050 | 1.264 |
| Rmeas | 0.053 | 1.320 |
| Rpim | 0.016 | 0.374 |
| Number of reflections | 39046 | 1029 |
| <I/σ(I)> | 25.5 | 2.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 11.8 | 12.3 |
| CC(1/2) | 0.999 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20%(v/v) PEG 4000, 10%(v/v) 2-propanol, 0.01M HEPES, pH 7.5 |
