5Z2E

Dipicolinate bound Dihydrodipicolinate reductase from Paenisporosarcina sp. TG-14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2017-10-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 64 2 2
Unit cell lengths	105.855, 105.855, 101.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.930 - 1.800
R-factor	0.16096
Rwork	0.159
R-free	0.19208
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.114
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	46.930
High resolution limit [Å]	1.800	1.800
Number of reflections	31600
<I/σ(I)>	89.2
Completeness [%]	100.0
Redundancy	43.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30%(v/v) PEG 400, 100mM HEPES:NaOH (pH 7.5), and 200mM MgCl2