5Z2D

Dihydrodipicolinate reductase from Paenisporosarcina sp. TG-14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2017-05-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 64 2 2
Unit cell lengths	106.229, 106.229, 101.999
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.110 - 1.800
R-factor	0.16861
Rwork	0.167
R-free	0.20677
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	2.132
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.847
High resolution limit [Å]	1.800	1.800
Number of reflections	31125
<I/σ(I)>	31.8
Completeness [%]	97.2
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30%(v/v) PEG 400, 100mM Tris-HCl (pH 8.5), and 200mM MgCl2