5Z28
AtVAL2 PHD-Like domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.558, 76.022, 76.046 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.882 - 2.245 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yug |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.110 | 2.330 |
| High resolution limit [Å] | 2.244 | 2.250 |
| Rmerge | 0.147 | |
| Number of reflections | 10184 | |
| <I/σ(I)> | 17.94 | |
| Completeness [%] | 99.6 | |
| Redundancy | 9.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris pH6.5, 28% PEG MME 2000 |
