5Z25
Trimeric Alpha-Helix-Inserted Circular Permutant of Cytochrome c555
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-29 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 153.804, 153.804, 153.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.22905 |
| Rwork | 0.227 |
| R-free | 0.26831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 19058 | 1005 |
| <I/σ(I)> | 23.839 | |
| Completeness [%] | 96.0 | 100 |
| Redundancy | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 2.0 M ammonium sulfate with 2% (v/v) PEG 400, 100 mM HEPES buffer |
