5Z1X
Crystal Structure of Laccase from Cerrena sp. RSD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-22 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.626, 88.795, 147.815 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.380 |
R-factor | 0.12719 |
Rwork | 0.126 |
R-free | 0.14948 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gyc |
RMSD bond length | 0.004 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.042 | 0.270 |
Number of reflections | 228137 | 21731 |
<I/σ(I)> | 44.2 | 4.8 |
Completeness [%] | 98.6 | 95 |
Redundancy | 4.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 1.4M ammonium sulfate, pH 7.6 |