5Z1X
Crystal Structure of Laccase from Cerrena sp. RSD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.626, 88.795, 147.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.380 |
| R-factor | 0.12719 |
| Rwork | 0.126 |
| R-free | 0.14948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.042 | 0.270 |
| Number of reflections | 228137 | 21731 |
| <I/σ(I)> | 44.2 | 4.8 |
| Completeness [%] | 98.6 | 95 |
| Redundancy | 4.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 1.4M ammonium sulfate, pH 7.6 |
