5Z16
A novel dimeric isocitrate dehydrogenase from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-11-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 137.161, 137.161, 238.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.537 - 3.000 |
| R-factor | 0.2082 |
| Rwork | 0.205 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mbc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 8.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.132 | 0.402 |
| Number of reflections | 46423 | 2297 |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES, 10% PEG 4000, 14% 2-propanol, 40mM MgCl2, 20% glycerol |
