5Z11
Crystal Structure of Grass Carp CD8 alpha alpha Homodimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-19 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.910, 47.910, 242.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.000 - 2.350 |
| R-factor | 0.20308 |
| Rwork | 0.200 |
| R-free | 0.25804 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 12041 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Ammonium phosphate monobasic, 20%(w/v) Polyethylene glycol 3,350 |
