5Z0V
Structural insight into the Zika virus capsid encapsulating the viral genome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.104, 223.076, 85.165 |
| Unit cell angles | 90.00, 109.99, 90.00 |
Refinement procedure
| Resolution | 46.363 - 2.913 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfk 1ez9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.752 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 46037 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 95.9 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 293 | 0.1 M sodium acetate trihydrate pH 4.6, 8% w/v Polyethylene glycol 4000 |
