5Z0R

Structural insight into the Zika virus capsid encapsulating the viral genome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97791
Spacegroup name	P 1 21 1
Unit cell lengths	74.301, 81.425, 77.190
Unit cell angles	90.00, 104.79, 90.00

Refinement procedure

Resolution	48.286 - 2.050
R-factor	0.2221
Rwork	0.221
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ez9 1sfk
RMSD bond length	0.004
RMSD bond angle	1.058
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Number of reflections	55737
<I/σ(I)>	10.5
Completeness [%]	94.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8.5	293	10% v/v 2-Propanol, 0.1 M BICINE pH 8.5, 30% w/v Polyethylene glycol 1500