5Z07

Crystal structure of centromere protein Cenp-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-08
Detector	RAYONIX MX225-HS
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	59.671, 59.671, 145.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.197 - 2.298
R-factor	0.1793
Rwork	0.174
R-free	0.22770
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.961
Data reduction software	AutoSol
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2463))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.298
Number of reflections	12379
<I/σ(I)>	21.4
Completeness [%]	99.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG4000