5Z06
Crystal structure of beta-1,2-glucanase from Parabacteroides distasonis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.080, 123.337, 101.376 |
| Unit cell angles | 90.00, 107.88, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.100 |
| R-factor | 0.17966 |
| Rwork | 0.178 |
| R-free | 0.21936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qt9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.570 |
| Number of reflections | 86780 | 4410 |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 3.9 | 2.8 |
| CC(1/2) | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 200mM calcium acetate, 100mM imidazole/ hydrochloric acid pH 8.0, and 20%(w/v) PEG 1000 |
