5Z01
Native Escherichia coli L,D-carboxypeptidase A (LdcA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-22 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.949, 89.069, 93.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.886 - 1.750 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.803 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 1.770 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.073 | 1.016 |
| Rpim | 0.370 | |
| Number of reflections | 33235 | 1801 |
| <I/σ(I)> | 19.4 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9 | 9.3 |
| CC(1/2) | 0.999 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.02M Tris pH 8.5, 0.2M sodium thiocyanate, 12% PEG 3350 |
