5YZD
Crystal structure of the prefusion form of measles virus fusion protein in complex with a fusion inhibitor peptide (FIP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-23 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 170.407, 170.407, 170.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.203 - 2.636 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yxw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.365 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11_2567)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.496 | 2.645 |
| High resolution limit [Å] | 2.636 | 2.636 |
| Number of reflections | 24414 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 21.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium formate, glycerol, sodium acetate |
