5YZA

Crystal Structure of Human CRMP-2 with S522D mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-09
Detector	RAYONIX MX225-HS
Wavelength(s)	1.00000
Spacegroup name	I 41 2 2
Unit cell lengths	112.420, 112.420, 195.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.841 - 2.300
R-factor	0.1921
Rwork	0.190
R-free	0.24500
RMSD bond length	0.008
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.083	0.698
Rmeas	0.089	0.748
Number of reflections	28103
<I/σ(I)>	27.5	3.1
Completeness [%]	99.9	99
Redundancy	7.7	7.8
CC(1/2)	0.999	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG 4000, 0.1M sodium citrate pH 5.6, 0.1M lithium sulfate