5YYU

Crystal structure of Staphylococcus aureus single-stranded DNA-binding protein SsbB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	298
Detector technology	CCD
Collection date	2017-11-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.975
Spacegroup name	P 21 21 21
Unit cell lengths	63.993, 84.739, 84.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.981 - 2.984
R-factor	0.2181
Rwork	0.214
R-free	0.29950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xgt
RMSD bond length	0.011
RMSD bond angle	1.385
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.090
High resolution limit [Å]	2.980	2.980
Number of reflections	9841
<I/σ(I)>	13.3	2.54
Completeness [%]	99.8	99.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	30% PEG 4000, 100mM Tris pH 8.5, 200mM Sodium Acetate