5YYN
Crystal structures of E.coli arginyl-trna synthetase (argrs) in complex with substrate TRNA(Arg)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03988 |
| Spacegroup name | P 65 |
| Unit cell lengths | 102.241, 102.241, 480.567 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.150 - 3.000 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bs2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.456 | |
| Number of reflections | 53802 | |
| <I/σ(I)> | 13.2 | 2.9 |
| Completeness [%] | 95.4 | 71.2 |
| Redundancy | 10.5 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 290 | 0.1M NA3C6H5O7, 1.95M (NH4)2SO4, PH 5.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 290K |
