5YYL

Structure of Major Royal Jelly Protein 1 Oligomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	H 3 2
Unit cell lengths	211.582, 211.582, 149.968
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.650
R-factor	0.241
Rwork	0.239
R-free	0.27500
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.464
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BUCCANEER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	37682
<I/σ(I)>	20.46
Completeness [%]	99.9
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Tris