5YXY

Crystal structure of the HyhL-HypA complex (form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2014-06-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1
Spacegroup name	C 2 2 2
Unit cell lengths	245.309, 261.427, 134.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.220 - 3.299
R-factor	0.3058
Rwork	0.305
R-free	0.32120
RMSD bond length	0.004
RMSD bond angle	0.925
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	3.500
High resolution limit [Å]	3.299	3.300
Rmerge	0.151	0.800
Number of reflections	64828
<I/σ(I)>	12.3
Completeness [%]	99.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1M NaCl, 0.1M MgCl2, 10% PEG 3350, 0.1M imidazole-HCl (pH 6.2)