5YXN
A T cell receptor in complex with HLA-A0201 restricted Hepatitis C virus NS3 peptide (KLVALGINAV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 79.990, 53.650, 224.981 |
| Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 |
| R-factor | 0.1944 |
| Rwork | 0.193 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.398 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 61621 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 21% PEG 3350, 0.18 M Ammonium tartrate dibasic |
