5YXM

Crystal structure of Chlamydomonas Outer Arm Dynein Light Chain 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	53.536, 69.230, 74.775
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.850 - 1.545
R-factor	0.1703
Rwork	0.169
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a9n 3ozx
RMSD bond length	0.008
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.545	1.545
Rmerge	0.094	0.946
Rmeas	0.098
Rpim	0.038	0.384
Number of reflections	41149	2043
<I/σ(I)>	36.49	3.22
Completeness [%]	99.8	100
Redundancy	7.1	7.1
CC(1/2)		0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 3350, ammonium phosphate monobasic