5YXC

Crystal structure of Zinc binding protein ZinT in complex with citrate from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9826
Spacegroup name	P 1 21 1
Unit cell lengths	41.040, 65.866, 73.536
Unit cell angles	90.00, 91.35, 90.00

Refinement procedure

Resolution	41.029 - 1.763
R-factor	0.1898
Rwork	0.188
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4aw8
RMSD bond length	0.011
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.029	1.790
High resolution limit [Å]	1.763	1.763
Number of reflections	37131	1828
<I/σ(I)>	0.1
Completeness [%]	90.5
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	288	22.5%(w/v) polyethylene glycol 4000, 0.135M ammonium acetate, and 0.1M sodium acetate trihydrate (pH 4.6)