5YWJ
Global regulatory element SarX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-22 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 0.97774 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 33.392, 33.392, 230.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.612 - 2.102 |
| R-factor | 0.2489 |
| Rwork | 0.247 |
| R-free | 0.28490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hs5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | xia2 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.087 | 0.221 |
| Number of reflections | 8345 | 557 |
| <I/σ(I)> | 8.19 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.16 | Bicine pH9.0, 1,4-Dioxane, PEG 20000 |
