5YUZ
DNA polymerase IV - DNA ternary complex 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.090, 57.130, 110.300 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 44.800 - 1.830 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ir1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.248 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.130 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.055 | 0.596 |
| Number of reflections | 94643 | |
| <I/σ(I)> | 12.5 | 2.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 15% MPD , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
