5YUO
Crystal structure of SSB protein from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2016-03-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.208, 64.104, 97.103 |
| Unit cell angles | 90.00, 112.96, 90.00 |
Refinement procedure
| Resolution | 26.490 - 2.037 |
| R-factor | 0.2196 |
| Rwork | 0.217 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.844 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.037 | 2.040 |
| Rmerge | 0.038 | 0.456 |
| Number of reflections | 36409 | 3312 |
| <I/σ(I)> | 31.36 | 2.53 |
| Completeness [%] | 98.2 | 89.9 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 14% PEG 4000, 100mM HEPES pH 7.0, 50mM sodium acetate |
